Search results for "Microchip Analytical Procedures"

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Biochips for cell biology by combined dip-pen nanolithography and DNA-directed protein immobilization.

2013

A general methodology for patterning of multiple protein ligands with lateral dimensions below those of single cells is described. It employs dip pen nanolithography (DPN) patterning of DNA oligonucleotides which are then used as capture strands for DNA-directed immobilization (DDI) of oligonucleotide-tagged proteins. This study reports the development and optimization of PEG-based liquid ink, used as carrier for the immobilization of alkylamino-labeled DNA oligomers on chemically activated glass surfaces. The resulting DNA arrays have typical spot sizes of 4-5 μm with a pitch of 12 μm micrometer. It is demonstrated that the arrays can be further functionalized with covalent DNA-streptavidi…

Materials scienceSurface PropertiesGreen Fluorescent ProteinsOligonucleotidesLigandsBiomaterialsCell membranechemistry.chemical_compoundEpidermal growth factorDip-pen nanolithographyCell Line TumorMaterials TestingMicrochip Analytical ProceduresmedicineHumansNanotechnologyGeneral Materials ScienceBiotinylationBiochipOligonucleotide Array Sequence AnalysisEpidermal Growth FactorOligonucleotideCell MembraneProteinsNanolitographyGeneral ChemistryCell BiologyDNABiochipCell biologymedicine.anatomical_structurecell.chemistryBiotinylationMCF-7 CellsGlassproteinDNABiotechnologyProtein ligandSmall (Weinheim an der Bergstrasse, Germany)

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PEPhysical Sciences and Engineering
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LSLife Sciences
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SHSocial Sciences and Humanities
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